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ZBTB8B

ANTIBODY INFORMATION
 
 

Antibody HPA027818

  

Antibody HPA028690

  

Antibody HPA055553

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA027818   HPA028690   HPA055553  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 6   6   13  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA027818

  

Antibody HPA028690

  

Antibody HPA055553

  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027818

  

Antibody HPA028690

  

Antibody HPA055553

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human duodenum shows distinct positivity in cytoplasm, membranes and nuclei of glandular cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in leydig cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in endocrine cells.  

Expression

 RNA: detected in 0 tissues
Protein: detected in 74 cell types		   RNA: detected in 0 tissues
Protein: detected in 53 cell types		   RNA: detected in 0 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:275   1:50   1:400  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA027818

  

Antibody HPA028690

  

Antibody HPA055553

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 54.2   54.2   54.2  

Antibody dilution

 1:250   1:250   1:270  

PROTEIN ARRAY
 
 

Antibody HPA027818

  

Antibody HPA028690

  

Antibody HPA055553

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:3000   1:2100  

ANTIGEN INFORMATION
 
 

Antibody HPA027818

  

Antibody HPA028690

  

Antibody HPA055553

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 70   96   76  

Antigen sequence

 
SVECLRESPCGDCGDCHPLELVVRDSLGGGSADSNLSTPPKRIEPKVEFD
ADEVEVDVGEQLQQYAAPLN
  
SGKLDLCGENVIEVMSAASYLQMNDVVNFCKTYIRSSLDICRKMEKEAAV
AAAVAAAAAAAAAAAAAAAHQVDSESPSSGREGTSCGTKSLVSSPA
  
SNRPIICKGCRRTFTSHLSQGLRRFGLCDSCTCVTDTPDDDDDLMPINLS
LVEASSESQEKSDTDNDWPIYVESEI
 

Matching transcripts

 ZBTB8B-001 - ENSP00000476499 [100%]
  ZBTB8B-001 - ENSP00000476499 [100%]
  ZBTB8B-001 - ENSP00000476499 [97%]
 

Other gene match

      
ANTIGEN VIEW
ZBTB8B-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.