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HNF1B

ANTIBODY INFORMATION
 
 

Antibody HPA002083

  

Antibody CAB068192

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002083   AMAb90733  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 2   13  

References

 5    

VALIDATION SUMMARY
 
 

Antibody HPA002083

  

Antibody CAB068192

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002083

  

Antibody CAB068192

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.    

Antibody dilution

 1:6    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002083

  

Antibody CAB068192

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong nuclear positivity in renal tubules.   Immunohistochemical staining of human kidney shows strong nuclear positivity in renal tubules.  

Expression

 RNA: detected in 20 tissues
Protein: detected in 19 cell types		   RNA: detected in 20 tissues
Protein: detected in 21 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002083

  

Antibody CAB068192

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400162)		    

Target mass (kDa)

 61.3, 60.2, 58.4, 50.3, 47.5, 32.7   61.3, 60.2, 58.4, 50.3, 47.5, 32.7  

Antibody dilution

 1:250   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA002083

  

Antibody CAB068192

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA002083

  

Antibody CAB068192

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 110    

Antigen sequence

 
KEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKG
RLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAA
KMIKGYMQQH
  

											 
																				

																		

										
											
Matching transcripts

												
																					HNF1B-001 - ENSP00000480291 [100%] 
HNF1B-003 - ENSP00000482711 [100%] 
HNF1B-004 - ENSP00000477524 [100%] 
HNF1B-005 - ENSP00000482529 [100%] 
HNF1B-201 - ENSP00000478682 [100%] 
HNF1B-202 - ENSP00000481245 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
HNF1B-001
HNF1B-003
HNF1B-004
HNF1B-005
HNF1B-201
HNF1B-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002083

												
										 
																				
											
											
											
Antibody CAB068192

												
										 
																		

																	

										
											
1.

												
																						Genomic profiling of papillary renal cell tumours identifies small regions of DNA alterations: a possible role of HNF1B in tumour development

	Szponar A et al

	Histopathology 2011;58(6):934-43

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Role of PINCH and its partner tumor suppressor Rsu-1 in regulating liver size and tumorigenesis

	Donthamsetty S et al

	PLoS One 2013;8(9):e74625

			
											 
																						
											 
																				

																		

										
											
3.

												
																						Hepatocyte nuclear factor-1β is not a specific marker of clear cell carcinoma in serous effusions

	Davidson B et al

	Cancer Cytopathol 2014;122(2):153-8

			Application: IHC				
											 
																						
											 
																				

																		

										
											
4.

												
																						Long-term primary culture of a clear cell ovarian carcinoma reveals an epithelial-mesenchymal cooperative interaction

	Goyeneche AA et al

	Cancer Cell Int 2015;15:88

			Application: IHC				
											 
																						
											 
																				

																		

										
											
5.

												
																						Prognostic relevance of molecular subtypes and master regulators in pancreatic ductal adenocarcinoma

	Janky R et al

	BMC Cancer 2016;16:632

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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