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Antibody staining overlaps with antibody HPA061823.
Validated
Antibody staining overlaps with antibody HPA016583.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:14
1:24
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA016583
Antibody HPA061823
Standard validation
Approved
Figure description
Immunohistochemical staining of human skin shows membranous positivity in squamous epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
Antibody dilution
1:20
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA016583
Antibody HPA061823
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403208)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
32.7, 26.1
32.7, 26.1
Antibody dilution
1:125
1:80
PROTEIN ARRAY
Antibody HPA016583
Antibody HPA061823
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
