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BX072566.1

ANTIBODY INFORMATION
 
 

Antibody HPA042822

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042822  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 POTED - ENSG00000166351 [98%]
POTEC - ENSG00000183206 [98%]
POTEG - ENSG00000187537 [95%]
POTEE - ENSG00000188219 [95%]
POTEF - ENSG00000196604 [95%]
POTEI - ENSG00000196834 [93%]
POTEH - ENSG00000198062 [95%]
POTEM - ENSG00000222036 [95%]
POTEJ - ENSG00000222038 [93%]
POTEB2 - ENSG00000230031 [98%]
POTEB - ENSG00000233917 [98%]
AC136352.4 - ENSG00000276760 [98%]
POTEB3 - ENSG00000278522 [98%]
  

Released in version

 10  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA042822

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042822

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human testis shows strong cytopolasmic positivity in cells in seminiferus ducts and leydig cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:1000  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042822

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 121.4, 121.3, 117.4, 66.4, 65.7, 61.7, 61.2, 61, 57.2, 57.1, 57, 56.9, 44.1, 42.9, 42.7, 42.6, 41.9, 41.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA042822

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA042822

  

Antigen

 Recombinant protein fragment  

Length (aa)

 41  

Antigen sequence

 
YDHSAFMEPRYHVRREDLDKLHRAAWWGKVPRKDLIVMLRD
 

Matching transcripts

 BX072566.1-201 - ENSP00000479139 [100%]
BX072566.1-202 - ENSP00000477677 [100%]
 

Other gene match

 POTED - ENSG00000166351 [98%]
POTEC - ENSG00000183206 [98%]
POTEG - ENSG00000187537 [95%]
POTEE - ENSG00000188219 [95%]
POTEF - ENSG00000196604 [95%]
POTEI - ENSG00000196834 [93%]
POTEH - ENSG00000198062 [95%]
POTEM - ENSG00000222036 [95%]
POTEJ - ENSG00000222038 [93%]
POTEB2 - ENSG00000230031 [98%]
POTEB - ENSG00000233917 [98%]
AC136352.4 - ENSG00000276760 [98%]
POTEB3 - ENSG00000278522 [98%]
  
ANTIGEN VIEW
BX072566.1-201
BX072566.1-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.