Fields »
ABOUT
HELP
BLOG
MPO

ANTIBODY INFORMATION
 
 

Antibody HPA021147

  

Antibody HPA061464

  

Antibody CAB000059

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
  

Product name

 HPA021147   HPA061464   A0398  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 5   13   1  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA021147

  

Antibody HPA061464

  

Antibody CAB000059

  
Immunocytochemistry

  Supported    
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA021147

  

Antibody HPA061464

  

Antibody CAB000059

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line SiHa shows localization to nucleus & vesicles.    

Antibody dilution

   1:66    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021147

  

Antibody HPA061464

  

Antibody CAB000059

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		      

 

 

      

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.      
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human bone marrow shows moderate positivity in hematopoietic cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.   Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in a subset of cells in red pulp.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 5 cell types		   RNA: detected in 9 tissues
Protein: detected in 7 cell types		   RNA: detected in 9 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH9  

Antibody dilution

 1:9000   1:3500   1:3000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA021147

  

Antibody HPA061464

  

Antibody CAB000059

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 83.9   83.9   83.9, 9.9  

Antibody dilution

 1:250   1:200   1:500  

PROTEIN ARRAY
 
 

Antibody HPA021147

  

Antibody HPA061464

  

Antibody CAB000059

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:2650    

ANTIGEN INFORMATION
 
 

Antibody HPA021147

  

Antibody HPA061464

  

Antibody CAB000059

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 140   42    

Antigen sequence

 
SRDHGLPGYNAWRRFCGLPQPETVGQLGTVLRNLKLARKLMEQYGTPNNI
DIWMGGVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWENEGVFSMQQ
RQALAQISLPRIICDNTGITTVSKNNIFMSNSYPRDFVNC
  
GAAPAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLR
   

Matching transcripts

 MPO-001 - ENSP00000225275 [100%]
  MPO-001 - ENSP00000225275 [100%]
   

Other gene match

      
ANTIGEN VIEW
MPO-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.