We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Antibody dilution
1:200
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA050843
Antibody CAB073533
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA050843
Antibody CAB073533
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400943)
Target mass (kDa)
37, 32, 31.6, 31.3, 25.2, 17.2, 17.1
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA050843
Antibody CAB073533
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
