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Antibody staining overlaps with antibody HPA028747.
Validated
Antibody staining overlaps with antibody HPA008763.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lysosomes.
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum, endosomes & lysosomes.
Antibody dilution
1:27
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA008763
Antibody HPA028747
Antibody CAB019394
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.
Expression
RNA: detected in 37 tissues Protein: detected in 57 cell types
RNA: detected in 37 tissues Protein: detected in 66 cell types
RNA: detected in 37 tissues Protein: detected in 24 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:50
1:100
1:600
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008763
Antibody HPA028747
Antibody CAB019394
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
