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ANTIBODY INFORMATION
 
 

Antibody HPA016939

  

Antibody HPA064668

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA016939   HPA064668  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA016939

  

Antibody HPA064668

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Uncertain    
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA016939

  

Antibody HPA064668

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA064668.		  
Validated

Antibody staining overlaps with antibody HPA016939.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.   Immunofluorescent staining of human cell line SK-MEL-30 shows localization to cytosol.  

Antibody dilution

 1:10   1:103  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016939

  

Antibody HPA064668

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human pancreas shows cytoplasmic positivity in both islet cells and exocrine glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:75    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA016939

  

Antibody HPA064668

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 61.3, 58.8   61.3, 58.8  

Antibody dilution

 1:250   1:340  

PROTEIN ARRAY
 
 

Antibody HPA016939

  

Antibody HPA064668

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:4100  

ANTIGEN INFORMATION
 
 

Antibody HPA016939

  

Antibody HPA064668

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 130   88  

Antigen sequence

 
NVLSFDDLTADALANLKVSQIKKVRLLIDEAILKCDAERIKLEAERFENL
REIGNLLHPSVPISNDEDVDNKVERIWGDCTVRKKYSHVDLVVMVDGFEG
EKGAVVAGSRGYFLKGVLVFLEQALIQYAL
  
GSRGYIPIYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEKSDDNSYDE
KYLIATSEQPIAALHRDEWLRPEDLPIKYAGLSTCFRQ
 

Matching transcripts

 SARS-001 - ENSP00000234677 [100%]
SARS-004 - ENSP00000358939 [100%]
  SARS-001 - ENSP00000234677 [100%]
SARS-004 - ENSP00000358939 [100%]
 

Other gene match

    
ANTIGEN VIEW
SARS-001
SARS-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.