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ARHGEF5

ANTIBODY INFORMATION
 
 

Antibody HPA020980

  

Antibody HPA029504

  

Antibody HPA044188

  

Antibody HPA045619

  

Antibody HPA045699

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020980   HPA029504   HPA044188   HPA045619   HPA045699  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

     ARHGEF35 - ENSG00000213214 [100%]
   ARHGEF35 - ENSG00000213214 [100%]
   ARHGEF35 - ENSG00000213214 [100%]
  

Released in version

 5   6   12   12   12  

References

          

VALIDATION SUMMARY
 
 

Antibody HPA020980

  

Antibody HPA029504

  

Antibody HPA044188

  

Antibody HPA045619

  

Antibody HPA045699

  
Immunocytochemistry


Validated
  

Validated
        
Immunohistochemistry

Approved   Approved   Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020980

  

Antibody HPA029504

  

Antibody HPA044188

  

Antibody HPA045619

  

Antibody HPA045699

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA029504.		  
Validated

Antibody staining overlaps with antibody HPA020980.		        
 

Standard validation

 Supported   Supported        

         

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.   Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & cytosol.        

Antibody dilution

 1:147   1:200        

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020980

  

Antibody HPA029504

  

Antibody HPA044188

  

Antibody HPA045619

  

Antibody HPA045699

  

Standard validation

 Approved   Approved   Approved   Approved   Approved  

         

Figure description

 Immunohistochemical staining of human hippocampus shows strong nuclear and cytoplasmic positivity in neurons.   Immunohistochemical staining of human duodenum show moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows moderate positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 77 cell types		   RNA: detected in 35 tissues
Protein: detected in 69 cell types		   RNA: detected in 35 tissues
Protein: detected in 33 cell types		   RNA: detected in 35 tissues
Protein: detected in 55 cell types		   RNA: detected in 35 tissues
Protein: detected in 40 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:200   1:500   1:250   1:350  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020980

  

Antibody HPA029504

  

Antibody HPA044188

  

Antibody HPA045619

  

Antibody HPA045699

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

         

Figure description

          

Target mass (kDa)

 176.8, 89.1   176.8, 60.1   176.8, 53.3   176.8, 53.3   176.8, 53.3  

Antibody dilution

 1:250   1:250   1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA020980

  

Antibody HPA029504

  

Antibody HPA044188

  

Antibody HPA045619

  

Antibody HPA045699

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:12000   1:6000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA020980

  

Antibody HPA029504

  

Antibody HPA044188

  

Antibody HPA045619

  

Antibody HPA045699

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 74   94   102   77   84  

Antigen sequence

 
PAREPLRRTTPQQGASGPGRSPVGQARQPEKPSHLHLEKASSWPHRRDSG
RPPGDSSGQAVAPSEGANKHKGWS
  
NLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNS
PQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDG
  
QGEERKRELQVPEENRADSQDEKSQTFLGKSEEVTGKQEDHGIKEKGVPV
SGQEAKEPESWDGGRLGAVGRARSREEENEHHGPSMPALIAPEDSPHCDL
FP
  
GSEEEEVEFWPGLTSLTLGSGQAEEEEETSSDNSGQTRYYSPCEEHPAET
NQNEGAESGTIRQGEELPSEELQESQG
  
HPQEVQVLEEQGQQEAGFRGEGTLREDVCADGLLGEEQMIEQVNDEKGEQ
KQKQEQVQDVMLGRQGERMGLTGEPEGLNDGEWE
 

Matching transcripts

 ARHGEF5-001 - ENSP00000056217 [100%]
ARHGEF5-002 - ENSP00000418745 [100%]
  ARHGEF5-001 - ENSP00000056217 [100%]
ARHGEF5-005 - ENSP00000418227 [100%]
  ARHGEF5-001 - ENSP00000056217 [100%]
  ARHGEF5-001 - ENSP00000056217 [97%]
  ARHGEF5-001 - ENSP00000056217 [100%]
 

Other gene match

     ARHGEF35 - ENSG00000213214 [100%]
   ARHGEF35 - ENSG00000213214 [100%]
   ARHGEF35 - ENSG00000213214 [100%]
  
ANTIGEN VIEW
ARHGEF5-001
ARHGEF5-002
ARHGEF5-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.