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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA038460

 

Antibody HPA038461

 

Antibody CAB075750

 

Antibody CAB075751

 

Antibody CAB075752

 

Antibody CAB075753

 

Antibody CAB075754

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA038460   HPA038461   AMAb91005   AMAb91006   AMAb91007   AMAb91008   AMAb91009  

Host species

Rabbit   Rabbit   Mouse   Mouse   Mouse   Mouse   Mouse  

Clonality

pAb   pAb   mAb   mAb   mAb   mAb   mAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G   Protein A/G   Protein A/G   Protein A/G  

Other gene match

             

Released in version

10   10   14   14   14   14   14  

References

             

VALIDATION SUMMARY

 
 

Antibody HPA038460

 

Antibody HPA038461

 

Antibody CAB075750

 

Antibody CAB075751

 

Antibody CAB075752

 

Antibody CAB075753

 

Antibody CAB075754

 
Immunocytochemistry


Validated

Validated
 

Validated
           
Immunohistochemistry

Supported   Supported   Supported   Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported   Supported   Supported   Supported  
Protein array

Supported   Supported            

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA038460

 

Antibody HPA038461

 

Antibody CAB075750

 

Antibody CAB075751

 

Antibody CAB075752

 

Antibody CAB075753

 

Antibody CAB075754

 

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.
             

0.81.01.30.31.90.50.70.90.31.50.40.60.81.01.21.41.61.8ScrambledsiRNA 1
 
0.81.01.30.31.90.50.70.90.31.50.40.60.81.01.21.41.61.8ScrambledsiRNA 1

Relative fluorescence intensity (RFI)

             

Figure description

Whole cells of segmented cells in 10x-images              

Antibody dilution

1:25              
 

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA038461.
 
Validated

Antibody staining overlaps with antibody HPA038460.
           
 

Standard validation

Supported   Supported            

             

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.            

Antibody dilution

1:25   1:62            

Literature conformity

The subcellular location is supported by literature.   The subcellular location is supported by literature.            

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA038460

 

Antibody HPA038461

 

Antibody CAB075750

 

Antibody CAB075751

 

Antibody CAB075752

 

Antibody CAB075753

 

Antibody CAB075754

 

Standard validation

Supported   Supported   Supported   Supported   Supported   Supported   Supported  

             

Figure description

Immunohistochemical staining of human colon shows strong cytoplasmic positivity, seen with a granular pattern, in glandular cells.   Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity with a granular pattern in glandular cells.   Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity with a granular pattern in glandular cells.  

Expression

RNA: detected in 37 tissues
Protein: detected in 72 cell types
  RNA: detected in 37 tissues
Protein: detected in 79 cell types
  RNA: detected in 37 tissues
Protein: detected in 76 cell types
  RNA: detected in 37 tissues
Protein: detected in 80 cell types
  RNA: detected in 37 tissues
Protein: detected in 75 cell types
  RNA: detected in 37 tissues
Protein: detected in 75 cell types
  RNA: detected in 37 tissues
Protein: detected in 79 cell types
 

Retrieval

HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

1:35   1:500   1:8000   1:8000   1:2500   1:6000   1:500  

Literature conformity

Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA038460

 

Antibody HPA038461

 

Antibody CAB075750

 

Antibody CAB075751

 

Antibody CAB075752

 

Antibody CAB075753

 

Antibody CAB075754

 

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
  Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
  Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
  Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
  Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
  Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
  Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
 

             

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

51.7, 50.4, 44.7   51.7, 50.4, 19.4, 16.3   51.7, 50.4, 44.7, 19.4, 17, 16.3, 16.1, 16, 15.8, 15.6, 15.5, 14.1, 13.3, 13.1, 12.6, 9.1, 7.4   51.7, 50.4, 44.7, 19.4, 17, 16.3, 16.1, 16, 15.8, 15.6, 15.5, 14.1, 13.3, 13.1, 12.6, 9.1, 7.4   51.7, 50.4, 44.7, 19.4, 17, 16.3, 16.1, 16, 15.8, 15.6, 15.5, 14.1, 13.3, 13.1, 12.6, 9.1, 7.4   51.7, 50.4, 44.7, 19.4, 17, 16.3, 16.1, 16, 15.8, 15.6, 15.5, 14.1, 13.3, 13.1, 12.6, 9.1, 7.4   51.7, 50.4, 44.7, 19.4, 17, 16.3, 16.1, 16, 15.8, 15.6, 15.5, 14.1, 13.3, 13.1, 12.6, 9.1, 7.4  

Antibody dilution

1:250   1:250   1:500   1:500   1:500   1:500   1:500  

PROTEIN ARRAY

 
 

Antibody HPA038460

 

Antibody HPA038461

 

Antibody CAB075750

 

Antibody CAB075751

 

Antibody CAB075752

 

Antibody CAB075753

 

Antibody CAB075754

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
  Supported

Pass with single peak corresponding to interaction only with its own antigen.
           

             

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.            

Antibody dilution

1:3000   1:3000            

ANTIGEN INFORMATION

 
 

Antibody HPA038460

 

Antibody HPA038461

 

Antibody CAB075750

 

Antibody CAB075751

 

Antibody CAB075752

 

Antibody CAB075753

 

Antibody CAB075754

 

Antigen

Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

91   100            

Antigen sequence

LDWSHNFTNMLGYTDHQFTELTRLYLTIHSDHEGGNVSAHTSHLVGSALS
DPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVS
 
ADLIPKEQARIKTFRQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDP
DEGIRFRGFSIPECQKLLPKAKGGEEPLPEGLFWLLVTGHIPTEEQVSWL
           

Matching transcripts

CS-001 - ENSP00000342056 [100%]
CS-002 - ENSP00000446779 [100%]
CS-003 - ENSP00000440543 [100%]
  CS-001 - ENSP00000342056 [100%]
CS-003 - ENSP00000440543 [100%]
CS-013 - ENSP00000448172 [100%]
CS-033 - ENSP00000447986 [96%]
           

Other gene match

             
ANTIGEN VIEW
CS-001
CS-002
CS-003
CS-013
CS-033
 

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.