ZFAT - Antibodies - The Human Protein Atlas

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ZFAT

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA020989

Antibody HPA063592

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA020989

HPA063592

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA020989

Antibody HPA063592

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA020989

Antibody HPA063592

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nuclear bodies.

Antibody dilution

1:175

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA020989

Antibody HPA063592

Standard validation

Supported

Figure description

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Expression

RNA: detected in 32 tissues
Protein: detected in 10 cell types

Retrieval

HIER pH6

Antibody dilution

1:600

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA020989

Antibody HPA063592

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Target mass (kDa)

139, 137.7, 132.1, 129.4, 128.7

139, 137.7, 132.1, 17.9

Antibody dilution

1:250

1:590

PROTEIN ARRAY

Antibody HPA020989

Antibody HPA063592

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:70000

ANTIGEN INFORMATION

Antibody HPA020989

Antibody HPA063592

Antigen

Recombinant protein fragment

Length (aa)

109

Antigen sequence

EPGGDIQQEALGDQLQLVEEEFALQGVNALKEEACPGDTQLEEGRKEPEA
PGEMPAPAVHLASPQAESTALPPCELETT

SESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTVVKQVTE
EEPSSNHTVMIQETVQQASVELAEQHHLVVSSDDVEGIETVTVYTQGGEA
SEFIVYVQE

Matching transcripts

ZFAT-001 - ENSP00000367069 [100%]
ZFAT-004 - ENSP00000429091 [100%]
ZFAT-005 - ENSP00000394501 [100%]
ZFAT-006 - ENSP00000428483 [100%]
ZFAT-007 - ENSP00000427831 [100%]
ZFAT-009 - ENSP00000427879 [100%]

ZFAT-001 - ENSP00000367069 [100%]
ZFAT-004 - ENSP00000429091 [100%]
ZFAT-006 - ENSP00000428483 [100%]
ZFAT-007 - ENSP00000427831 [100%]
ZFAT-015 - ENSP00000429600 [96%]

Other gene match

ANTIGEN VIEW

ZFAT-001	ZFAT-004	ZFAT-005	ZFAT-006	ZFAT-007	ZFAT-009	ZFAT-015

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.