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TNFRSF1A

ANTIBODY INFORMATION
 
 

Antibody HPA004102

  

Antibody CAB010309

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA004102   sc-7895  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 4   3  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA004102

  

Antibody CAB010309

  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004102

  

Antibody CAB010309

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 61 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:25  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004102

  

Antibody CAB010309

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 50.5, 33.2, 28.1, 24.2   50.5, 45.7, 33.2, 28.1, 24.2, 12.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004102

  

Antibody CAB010309

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA004102

  

Antibody CAB010309

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 148    

Antigen sequence

 
GDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCREC
ESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRH
YWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSC
  

											 
																				

																		

										
											
Matching transcripts

												
																					TNFRSF1A-001 - ENSP00000162749 [100%] 
TNFRSF1A-004 - ENSP00000380389 [100%] 
TNFRSF1A-014 - ENSP00000442059 [100%] 
TNFRSF1A-013 - ENSP00000413224 [84%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TNFRSF1A-001
TNFRSF1A-004
TNFRSF1A-013
TNFRSF1A-014
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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