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TP53BP1

ANTIBODY INFORMATION
 
 

Antibody HPA008788

  

Antibody HPA022133

  

Antibody CAB004083

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Cell Signaling Technology, Inc
  

Product name

 HPA008788   HPA022133   4937  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 3   5   2  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA008788

  

Antibody HPA022133

  

Antibody CAB004083

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008788

  

Antibody HPA022133

  

Antibody CAB004083

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies.    

Antibody dilution

 1:50   1:50    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008788

  

Antibody HPA022133

  

Antibody CAB004083

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows distinct nuclear positivity in seminiferus duct cells.   Immunohistochemical staining of human cerebellum shows strong nuclear positivity.   Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 53 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:40   1:400   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008788

  

Antibody HPA022133

  

Antibody CAB004083

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 214.1, 214, 213.6, 209   214.1, 214, 213.6, 209, 111.7   214.1, 214, 213.6, 209, 111.7, 16.4, 15.1  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008788

  

Antibody HPA022133

  

Antibody CAB004083

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:750    

ANTIGEN INFORMATION
 
 

Antibody HPA008788

  

Antibody HPA022133

  

Antibody CAB004083

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 139   105    

Antigen sequence

 
AYQCLLIADQHCRTRKYFLCLASGIPCVSHVWVHDSCHANQLQNYRNYLL
PAGYSLEEQRILDWQPRENPFQNLKVLLVSDQQQNFLELWSEILMTGGAA
SVKQHHSSAHNKDIALGVFDVVVTDPSCPASVLKCAEAL
  
VPSPATRSEALSSVLDQEEAMEIKEHHPEEGSSGSEVEEIPETPCESQGE
ELKEENMESVPLHLSLTETQSQGLCLQKEMPKKECSEAMEVETSVISIDS
PQKLA
  

											 
																				

																		

										
											
Matching transcripts

												
																					TP53BP1-001 - ENSP00000371475 [100%] 
TP53BP1-002 - ENSP00000263801 [100%] 
TP53BP1-004 - ENSP00000371470 [100%] 
TP53BP1-014 - ENSP00000393497 [100%] 

											 
																						TP53BP1-001 - ENSP00000371475 [100%] 
TP53BP1-002 - ENSP00000263801 [100%] 
TP53BP1-004 - ENSP00000371470 [100%] 
TP53BP1-005 - ENSP00000388028 [100%] 
TP53BP1-014 - ENSP00000393497 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TP53BP1-001
TP53BP1-002
TP53BP1-004
TP53BP1-005
TP53BP1-014
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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