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Antibody staining overlaps with antibody HPA024596.
Validated
Antibody staining overlaps with antibody HPA024594.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Antibody dilution
1:200
1:10
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA024212
Antibody HPA024594
Antibody HPA024596
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA024596 across 46 cell lines. Pearson correlation with HPA024594 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA024596 across 46 cell lines. Pearson correlation with HPA024212 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA024594 across 46 cell lines. Pearson correlation with HPA024212 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA024596 across 72 cell types. Spearman correlation with HPA024594 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA024596 across 73 cell types. Spearman correlation with HPA024212 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA024594 across 73 cell types. Spearman correlation with HPA024212 across 72 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity with a granular pattern in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 64 cell types
RNA: detected in 37 tissues Protein: detected in 63 cell types
RNA: detected in 37 tissues Protein: detected in 42 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:400
1:400
1:10
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA024212
Antibody HPA024594
Antibody HPA024596
Formal validation: Genetic
Validated
Downregulation visible in one of two siRNA lanes
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: siRNA 1 Lane 3: siRNA 2 Lane 4: Scrambled
