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Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 4 tissues Protein: detected in 37 cell types
RNA: detected in 4 tissues Protein: detected in 74 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:50
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031433
Antibody CAB009660
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
111
111, 53.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA031433
Antibody CAB009660
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
