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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:85
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA035640
Antibody CAB037313
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic and nucleolar positivity in neuronal cells.
Expression
RNA: detected in 34 tissues Protein: detected in 31 cell types
RNA: detected in 34 tissues Protein: detected in 11 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:3000
1:10000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035640
Antibody CAB037313
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400522)
Target mass (kDa)
74.3, 62.2, 62
74.3, 62.2, 62
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA035640
Antibody CAB037313
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
