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ANTIBODY INFORMATION
 
 

Antibody HPA039412

  

Antibody HPA047958

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039412   HPA047958  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   10  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA039412

  

Antibody HPA047958

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039412

  

Antibody HPA047958

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line RT4 shows localization to cytosol & mitochondria.  

Antibody dilution

   1:56  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039412

  

Antibody HPA047958

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity.   Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in gastric parietal cells.  

Expression

 RNA: detected in 29 tissues
Protein: detected in 63 cell types		   RNA: detected in 29 tissues
Protein: detected in 32 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:175  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039412

  

Antibody HPA047958

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 98.1   98.1  

Antibody dilution

 1:250   1:280  

PROTEIN ARRAY
 
 

Antibody HPA039412

  

Antibody HPA047958

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2250  

ANTIGEN INFORMATION
 
 

Antibody HPA039412

  

Antibody HPA047958

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 117   112  

Antigen sequence

 
VSTSPLDLSAHQADFVPISFYKIFGFPTGLGALLVHNRAAPLLRKTYFGG
GTASAYLAGEDFYIPRQSVAQRFEDGTISFLDVIALKHGFDTLERLTGGM
ENIKQHTFTLAQYTYVA
  
NQGLLYDRSWMVVNHNGVCLSQKQEPRLCLIQPFIDLRQRIMVIKAKGME
PIEVPLEENSERTQIRQSRVCADRVSTYDCGEKISSWLSTFFGRPCHLIK
QSSNSQRNAKKK
 

Matching transcripts

 MOCOS-001 - ENSP00000261326 [100%]
  MOCOS-001 - ENSP00000261326 [100%]
 

Other gene match

    
ANTIGEN VIEW
MOCOS-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.