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TYR

ANTIBODY INFORMATION
 
 

Antibody HPA043241

  

Antibody HPA050889

  

Antibody CAB000079

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA043241   HPA050889   NCL-L-TYROS  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 16   16   1  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA043241

  

Antibody HPA050889

  

Antibody CAB000079

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

   

Validated
  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043241

  

Antibody HPA050889

  

Antibody CAB000079

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA050889.		  
Validated

Antibody staining overlaps with antibody HPA043241.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line SK-MEL-30 shows localization to vesicles.   Immunofluorescent staining of human cell line SK-MEL-30 shows localization to vesicles.    

Antibody dilution

 1:49   1:192    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043241

  

Antibody HPA050889

  

Antibody CAB000079

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.  
 

Standard validation

     Supported  

     

Figure description

     Immunohistochemical staining of human skin shows strong cyoplasmic positivity in melanocytes.  

Expression

     RNA: detected in 1 tissues
Protein: detected in 2 cell types		  

Retrieval

     HIER pH9  

Antibody dilution

     1:100  

Literature conformity

     Consistent with extensive gene/protein characterization data.  

RNA consistency

     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043241

  

Antibody HPA050889

  

Antibody CAB000079

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 60.4   60.4   60.4  

Antibody dilution

 1:250   1:960   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043241

  

Antibody HPA050889

  

Antibody CAB000079

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:7700    

ANTIGEN INFORMATION
 
 

Antibody HPA043241

  

Antibody HPA050889

  

Antibody CAB000079

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 59   32    

Antigen sequence

 
APIGHNRESYMVPFIPLYRNGDFFISSKDLGYDYSYLQDSDPDSFQDYIK
SYLEQASRI
  
LLCRHKRKQLPEEKQPLLMEKEDYHSLYQSHL
   

Matching transcripts

 TYR-001 - ENSP00000263321 [100%]
  TYR-001 - ENSP00000263321 [100%]
   

Other gene match

      
ANTIGEN VIEW
TYR-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.