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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Antibody dilution
1:200
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA068792
Standard validation
Supported
Figure description
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts and weak nuclear positivity in Leydig cells.
Expression
RNA: detected in 36 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
Antibody dilution
1:2000
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA068792
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
68.2
Antibody dilution
1:780
PROTEIN ARRAY
Antibody HPA068792
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
