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SDF4

ANTIBODY INFORMATION
 
 

Antibody HPA011249

  

Antibody CAB015227

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA011249   2255  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 11   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA011249

  

Antibody CAB015227

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA011249

  

Antibody CAB015227

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.  

Antibody dilution

 1:26   1:125  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA011249

  

Antibody CAB015227

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 43 cell types		   RNA: detected in 37 tissues
Protein: detected in 22 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:1500  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA011249

  

Antibody CAB015227

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 41.8, 39.6   41.8, 39.6, 28  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA011249

  

Antibody CAB015227

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA011249

  

Antibody CAB015227

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 73    

Antigen sequence

 
DQDGDKQLSVPEFISLPVGTVENQQGQDIDDNWVKDRKKEFEELIDSNHD
GIVTAEEPQSYMDLRRAPPLSNN
   

Matching transcripts

 SDF4-001 - ENSP00000353094 [84%]
SDF4-007 - ENSP00000263741 [81%]
   

Other gene match

    
ANTIGEN VIEW
SDF4-001
SDF4-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.