OXCT1 - Antibodies - The Human Protein Atlas

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OXCT1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA012047

Antibody HPA061425

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA012047

HPA061425

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA012047

Antibody HPA061425

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA012047

Antibody HPA061425

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

Immunofluorescent staining of human cell line SiHa shows localization to mitochondria.

Antibody dilution

1:250

1:53

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA012047

Antibody HPA061425

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 41 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA061425 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA012047 across 72 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemical staining of human kidney shows cytoplasmic positivity in tubules.

Expression

RNA: detected in 36 tissues
Protein: detected in 59 cell types

RNA: detected in 36 tissues
Protein: detected in 58 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

1:400

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA012047

Antibody HPA061425

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

56.2

Antibody dilution

1:250

1:180

PROTEIN ARRAY

Antibody HPA012047

Antibody HPA061425

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:2100

ANTIGEN INFORMATION

Antibody HPA012047

Antibody HPA061425

Antigen

Recombinant protein fragment

Length (aa)

147

Antigen sequence

VGGFGLCGIPENLIDALLKTGVKGLTAVSNNAGVDNFGLGLLLRSKQIKR
MVSSYVGENAEFERQYLSGELEVELTPQGTLAERIRAGGAGVPAFYTPTG
YGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDF

ATWYKGCVCSFSTSAHRHTKFYTDPVEAVKDIPDGA

Matching transcripts

OXCT1-001 - ENSP00000196371 [100%]

Other gene match

ANTIGEN VIEW

OXCT1-001

RELEVANT PUBLICATIONS

Antibody HPA012047

Antibody HPA061425

Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy
Chang HT et al
Nutr Metab (Lond) 2013;10(1):47

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.