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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Antibody dilution
1:59
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA019357
Antibody HPA020131
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong nuclear positivity.
Expression
RNA: detected in 37 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019357
Antibody HPA020131
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404274)
Target mass (kDa)
35, 33.4, 33.1, 27.7, 23.8
35, 33.4, 33.1, 22.8
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA019357
Antibody HPA020131
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
