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SLC25A24

ANTIBODY INFORMATION
 
 

Antibody HPA028519

  

Antibody HPA063636

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA028519   HPA063636  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA028519

  

Antibody HPA063636

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

  Supported  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028519

  

Antibody HPA063636

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.   Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.  

Antibody dilution

 1:200   1:73  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028519

  

Antibody HPA063636

  

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity with a granular pattern in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:300  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028519

  

Antibody HPA063636

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 53.4, 51.4   53.4, 7.7  

Antibody dilution

 1:250   1:240  

PROTEIN ARRAY
 
 

Antibody HPA028519

  

Antibody HPA063636

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2900  

ANTIGEN INFORMATION
 
 

Antibody HPA028519

  

Antibody HPA063636

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 84   75  

Antigen sequence

 
SLWRGNGTNVIKIAPETAVKFWAYEQYKKLLTEEGQKIGTFERFISGSMA
GATAQTFIYPMEVMKTRLAVGKTGQYSGIYDCAK
  
MLRWLRDFVLPTAACQDAEQPTRYETLFQALDRNGDGVVDIGELQEGLRN
LGIPLGQDAEEKIFTTGDVNKDGKL
 

Matching transcripts

 SLC25A24-001 - ENSP00000457733 [100%]
SLC25A24-002 - ENSP00000359058 [100%]
  SLC25A24-001 - ENSP00000457733 [100%]
SLC25A24-004 - ENSP00000454393 [81%]
 

Other gene match

    
ANTIGEN VIEW
SLC25A24-001
SLC25A24-002
SLC25A24-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.