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ANTIBODY INFORMATION
 
 

Antibody HPA008759

  

Antibody HPA012602

  

Antibody CAB009403

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA008759   HPA012602   sc-20135  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 4   4   3  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA008759

  

Antibody HPA012602

  

Antibody CAB009403

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

Supported  

Validated
  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008759

  

Antibody HPA012602

  

Antibody CAB009403

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.   Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.    

Antibody dilution

 1:50   1:43    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008759

  

Antibody HPA012602

  

Antibody CAB009403

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in medullary cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.   Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in medullary cells.  

Expression

 RNA: detected in 27 tissues
Protein: detected in 44 cell types		   RNA: detected in 27 tissues
Protein: detected in 26 cell types		   RNA: detected in 27 tissues
Protein: detected in 26 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:250   1:600  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  
 

Standard validation

   Supported    

     

Figure description

   Synaptic staining in many regions of the brain.    

WESTERN BLOT
 
 

Antibody HPA008759

  

Antibody HPA012602

  

Antibody CAB009403

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

      

Target mass (kDa)

 78.3   78.3   78.3, 32.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008759

  

Antibody HPA012602

  

Antibody CAB009403

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA008759

  

Antibody HPA012602

  

Antibody CAB009403

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 123   135    

Antigen sequence

 
KQYSSHHTAEKRKRLGELFNPYYDPLQWKSSHFERRDNMNDNFLEGEEEN
ELTLNEKNFFPEYNYDWWEKKPFSEDVNWGYEKRNLARVPKLDLKRQYDR
VAQLDQLLHYRKKSAEFPDFYDS
  
SEVDKRRTRPRHHHGRSRPDRSSQGGSLPSEEKGHPQEESEESNVSMASL
GEKRDHHSTHYRASEEEPEYGEEIKGYPGVQAPEDLEWERYRGRGSEEYR
APRPQSEESWDEEDKRNYPSLELDKMAHGYGEESE
   

Matching transcripts

 CHGB-001 - ENSP00000368244 [100%]
  CHGB-001 - ENSP00000368244 [100%]
   

Other gene match

      
ANTIGEN VIEW
CHGB-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.