NOS1 - Antibodies - The Human Protein Atlas

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NOS1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA058312

Antibody CAB002167

Provider

Atlas Antibodies
Sigma-Aldrich

Novocastra

Product name

HPA058312

NCL-NOS-1

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA058312

Antibody CAB002167

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA058312

Antibody CAB002167

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.

Antibody dilution

1:123

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA058312

Antibody CAB002167

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.

Expression

RNA: detected in 8 tissues
Protein: detected in 3 cell types

Retrieval

HIER pH6

Antibody dilution

1:10

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA058312

Antibody CAB002167

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Target mass (kDa)

164.8, 161

Antibody dilution

1:410

1:500

PROTEIN ARRAY

Antibody HPA058312

Antibody CAB002167

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:4950

ANTIGEN INFORMATION

Antibody HPA058312

Antibody CAB002167

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

LVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIR
VTQPLGPPTKAVDLSHQPPAGKEQPLAV

Matching transcripts

NOS1-001 - ENSP00000320758 [100%]
NOS1-002 - ENSP00000337459 [100%]
NOS1-201 - ENSP00000339862 [100%]
NOS1-202 - ENSP00000477999 [100%]

Other gene match

ANTIGEN VIEW

NOS1-001	NOS1-002	NOS1-201	NOS1-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.