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IFT74

ANTIBODY INFORMATION
 
 

Antibody HPA020247

  

Antibody HPA026684

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020247   HPA026684  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA020247

  

Antibody HPA026684

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020247

  

Antibody HPA026684

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.    

Antibody dilution

 1:20    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020247

  

Antibody HPA026684

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human fallopian tube shows strong membranous positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 38 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:20   1:275  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020247

  

Antibody HPA026684

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 69.2, 42.3   69.2  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA020247

  

Antibody HPA026684

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA020247

  

Antibody HPA026684

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 99   103  

Antigen sequence

 
ELQGQLADYNMLVDKLNTNTEMEEVMNDYNMLKAQNDRETQSLDVIFTER
QAKEKQIRSVEEEIEQEKQATDDIIKNMSFENQVKYLEMKTTNEKLLQE
  
KIKKLHQERMILSTHRNAFKKIMEKQNIEYEALKTQLQENETHSQLTNLE
RKWQHLEQNNFAMKEFIATKSQESDYQPIKKNVTKQIAEYNKTIVDALHS
TSG
 

Matching transcripts

 IFT74-001 - ENSP00000369402 [100%]
IFT74-004 - ENSP00000393907 [100%]
IFT74-201 - ENSP00000389224 [100%]
IFT74-202 - ENSP00000404122 [100%]
  IFT74-001 - ENSP00000369402 [100%]
IFT74-201 - ENSP00000389224 [100%]
IFT74-202 - ENSP00000404122 [100%]
 

Other gene match

    
ANTIGEN VIEW
IFT74-001
IFT74-004
IFT74-201
IFT74-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.