TRIOBP - Antibodies - The Human Protein Atlas

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TRIOBP

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA003747

Antibody HPA019769

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA003747

HPA019769

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA003747

Antibody HPA019769

Immunocytochemistry

Uncertain

Immunohistochemistry

Western blot

Uncertain

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA003747

Antibody HPA019769

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Antibody dilution

1:100

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA003747

Antibody HPA019769

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA019769 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA003747 across 72 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 61 cell types

RNA: detected in 37 tissues
Protein: detected in 68 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

1:400

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA003747

Antibody HPA019769

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

261.4, 74

261.4, 74, 47.6

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA003747

Antibody HPA019769

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA003747

Antibody HPA019769

Antigen

Recombinant protein fragment

Length (aa)

134

101

Antigen sequence

EIGALMRQAEEREHTLRRCQQEGQELLRHNQELHGRLSEEIDQLRGFIAS
QGMGNGCGRSNERSSCELEVLLRVKENELQYLKKEVQCLRDELQMMQKDK
RFTSGKYQDVYVELSHIKTRSEREIEQLKEHLRL

ATDSRTPEVPAGEGPRRGLGAPLTEDQQNRLSEEIEKKWQELEKLPLREN
KRVPLTALLNQSRGERRGPPSDGHEALEKEVQALRAQLEAWRLQGEAPQS
A

Matching transcripts

TRIOBP-001 - ENSP00000384312 [100%]
TRIOBP-006 - ENSP00000386026 [100%]

TRIOBP-001 - ENSP00000384312 [100%]
TRIOBP-005 - ENSP00000383913 [100%]
TRIOBP-006 - ENSP00000386026 [100%]

Other gene match

ANTIGEN VIEW

TRIOBP-001	TRIOBP-005	TRIOBP-006

RELEVANT PUBLICATIONS

Antibody HPA003747

Antibody HPA019769

Aggregation of the protein TRIOBP-1 and its potential relevance to schizophrenia
Bradshaw NJ et al
PLoS One 2014;9(10):e111196
Application: ICC-IF, WB

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.