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Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:21
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA001581
Antibody HPA001790
Standard validation
Approved
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic and membranous positivity in bile duct cells.
Expression
RNA: detected in 34 tissues Protein: detected in 28 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001581
Antibody HPA001790
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
58.8, 49
58.8, 49
Antibody dilution
1:500
1:500
PROTEIN ARRAY
Antibody HPA001581
Antibody HPA001790
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
