RAB11A - Antibodies - The Human Protein Atlas

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RAB11A

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA051697

Antibody HPA060686

Antibody CAB013097

Provider

Atlas Antibodies
Sigma-Aldrich

Zymed

Product name

HPA051697

HPA060686

71-5300

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA051697

Antibody HPA060686

Antibody CAB013097

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA051697

Antibody HPA060686

Antibody CAB013097

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to microtubule organizing center & vesicles.

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubule organizing center.

Antibody dilution

1:51

1:50

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA051697

Antibody HPA060686

Antibody CAB013097

Standard validation

Approved

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 52 cell types

RNA: detected in 37 tissues
Protein: detected in 74 cell types

Retrieval

HIER pH6

Antibody dilution

1:60

1:200

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA051697

Antibody HPA060686

Antibody CAB013097

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

24.4, 22.5, 16.5

24.4, 22.5, 17.7, 16.5, 9.2

Antibody dilution

1:260

1:130

1:500

PROTEIN ARRAY

Antibody HPA051697

Antibody HPA060686

Antibody CAB013097

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2050

1:1550

ANTIGEN INFORMATION

Antibody HPA051697

Antibody HPA060686

Antibody CAB013097

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

RRENDMSPSNNVVPIHVPPTTENKPKVQ

RRENDMSPSNNVVPIHVPPTTENKPKVQ

Matching transcripts

RAB11A-001 - ENSP00000261890 [100%]
RAB11A-002 - ENSP00000455567 [100%]
RAB11A-004 - ENSP00000456638 [100%]

Other gene match

ANTIGEN VIEW

RAB11A-001	RAB11A-002	RAB11A-004

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.