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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Antibody dilution
1:72
1:56
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA040395
Antibody CAB010246
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human lymph node shows distinct nuclear positivity in non-germinal center cells.
Immunohistochemical staining of human lymph node shows distinct nuclear positivity in non-germinal center cells.
Expression
RNA: detected in 33 tissues Protein: detected in 53 cell types
RNA: detected in 33 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:225
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040395
Antibody CAB010246
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
82
82, 5.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA040395
Antibody CAB010246
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
