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GLI3

ANTIBODY INFORMATION
 
 

Antibody HPA005534

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA005534  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA005534

  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005534

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 37 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:40  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005534

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 169.9  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA005534

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000  

ANTIGEN INFORMATION
 
 

Antibody HPA005534

  

Antigen

 Recombinant protein fragment  

Length (aa)

 144  

Antigen sequence

 
GQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFG
GSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYS
GQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGA
 

Matching transcripts

 GLI3-001 - ENSP00000379258 [100%]
 

Other gene match

  
ANTIGEN VIEW
GLI3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.