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Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in granular pattern in glial and neuronal cells.
Immunohistochemical staining of human epididymis shows strong cytoplasmic and nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 34 cell types
RNA: detected in 37 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004938
Antibody CAB009916
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400343)
Target mass (kDa)
21, 13.7
21.1, 21, 13.7, 6.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA004938
Antibody CAB009916
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
