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TRDMT1

ANTIBODY INFORMATION
 
 

Antibody HPA036945

  

Antibody HPA036946

  

Antibody CAB009468

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA036945   HPA036946   sc-20702  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   12   3  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA036945

  

Antibody HPA036946

  

Antibody CAB009468

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036945

  

Antibody HPA036946

  

Antibody CAB009468

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.    

Antibody dilution

   1:68    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036945

  

Antibody HPA036946

  

Antibody CAB009468

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human colon shows cytoplasmic and nuclear positivity in glandular cells.   Immunohistochemical staining of human stomach, lower shows strong cytoplasmic and additional nucleolar positivity in subset of glandular cells.   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 73 cell types		   RNA: detected in 33 tissues
Protein: detected in 33 cell types		   RNA: detected in 33 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:150   1:100  

Literature conformity

 Partly consistent with gene/protein characterization data.   Not consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036945

  

Antibody HPA036946

  

Antibody CAB009468

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 44.6   44.6, 28.9, 25.8   44.6, 28.9, 25.8, 15.6, 9.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036945

  

Antibody HPA036946

  

Antibody CAB009468

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA036945

  

Antibody HPA036946

  

Antibody CAB009468

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 92   91    

Antigen sequence

 
RVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLA
KTIEGITLEEFDRLSFDMILMSPPCQPFTRIGRQGDMTDSRT
  
KIESVHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLETAEE
IHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYAL
  

											 
																				

																		

										
											
Matching transcripts

												
																					TRDMT1-001 - ENSP00000367030 [100%] 

											 
																						TRDMT1-001 - ENSP00000367030 [100%] 
TRDMT1-003 - ENSP00000324263 [100%] 
TRDMT1-005 - ENSP00000419625 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TRDMT1-001
TRDMT1-003
TRDMT1-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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