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PTGES3

ANTIBODY INFORMATION
 
 

Antibody HPA038672

  

Antibody HPA038673

  

Antibody CAB034319

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA038672   HPA038673   sc-101496  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 7   7   8  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA038672

  

Antibody HPA038673

  

Antibody CAB034319

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038672

  

Antibody HPA038673

  

Antibody CAB034319

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA038673.		  
Validated

Antibody staining overlaps with antibody HPA038672.		    
 

Standard validation

 Approved   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.    

Antibody dilution

 1:6   1:86    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038672

  

Antibody HPA038673

  

Antibody CAB034319

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferous ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 56 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		   RNA: detected in 37 tissues
Protein: detected in 30 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:35   1:500   1:100  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038672

  

Antibody HPA038673

  

Antibody CAB034319

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 19.2, 18.7, 14.8, 14.6   19.2, 18.7, 16.5, 15   19.2, 18.7, 16.5, 15, 14.8, 14.6  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038672

  

Antibody HPA038673

  

Antibody CAB034319

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA038672

  

Antibody HPA038673

  

Antibody CAB034319

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 63   71    

Antigen sequence

 
KERAKLNWLSVDFNNWKDWEDDSDEDMSNFDRFSEMMNNMGGDEDVDLPE
VDGADDDSQDSDD
  
CVEDSKDVNVNFEKSKLTFSCLGGSDNFKHLNEIDLFHCIDPNDSKHKRT
DRSILCCLRKGESGQSWPRLT
  

											 
																				

																		

										
											
Matching transcripts

												
																					PTGES3-001 - ENSP00000262033 [100%] 
PTGES3-002 - ENSP00000389090 [100%] 
PTGES3-201 - ENSP00000482075 [100%] 
PTGES3-006 - ENSP00000402385 [92%] 

											 
																						PTGES3-001 - ENSP00000262033 [100%] 
PTGES3-003 - ENSP00000405299 [100%] 
PTGES3-004 - ENSP00000414892 [100%] 
PTGES3-201 - ENSP00000482075 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PTGES3-001
PTGES3-002
PTGES3-003
PTGES3-004
PTGES3-006
PTGES3-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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