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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA056940 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA051452 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA056940 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA051452 across 74 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
Expression
RNA: detected in 37 tissues Protein: detected in 38 cell types
RNA: detected in 37 tissues Protein: detected in 32 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:850
1:200
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051452
Antibody HPA056940
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
76.3, 71.6, 71.4, 41.4
76.3, 71.6, 71.4, 36.5
Antibody dilution
1:110
1:130
PROTEIN ARRAY
Antibody HPA051452
Antibody HPA056940
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:900
1:1500
ANTIGEN INFORMATION
Antibody HPA051452
Antibody HPA056940
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
88
Antigen sequence
PSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQ
AVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLW
P
