Fields »
ABOUT
HELP
BLOG
EDEM3

ANTIBODY INFORMATION
 
 

Antibody HPA025754

  

Antibody HPA025755

  

Antibody HPA027297

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA025754   HPA025755   HPA027297  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   5   16  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA025754

  

Antibody HPA025755

  

Antibody HPA027297

  
Immunocytochemistry

    Approved  
Immunohistochemistry

Approved   Approved    
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA025754

  

Antibody HPA025755

  

Antibody HPA027297

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.  

Antibody dilution

     1:84  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA025754

  

Antibody HPA025755

  

Antibody HPA027297

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 34 cell types		   RNA: detected in 37 tissues
Protein: detected in 64 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:10   1:8    

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA025754

  

Antibody HPA025755

  

Antibody HPA027297

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 104.7, 102   104.7, 102   104.7, 102  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA025754

  

Antibody HPA025755

  

Antibody HPA027297

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA025754

  

Antibody HPA025755

  

Antibody HPA027297

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 81   100   109  

Antigen sequence

 
YLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNQSISKKNTTSEYTE
LDDSNFDWTCPNTQILFPNDPLYAQSIREPL
  
FRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAA
SSIDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGP
  
SSQEVDLVDQESSEENSLNSHPESLSLADMDNAASISPSEQTSNPTENHE
TTNLNGECTDLDNQLQEQSETEEDSNPNVSWGKKVQPIDSILADWNEDIE
AFEMMEKDE
 

Matching transcripts

 EDEM3-001 - ENSP00000318147 [100%]
EDEM3-201 - ENSP00000356482 [100%]
  EDEM3-001 - ENSP00000318147 [100%]
EDEM3-201 - ENSP00000356482 [100%]
  EDEM3-001 - ENSP00000318147 [100%]
EDEM3-201 - ENSP00000356482 [100%]
 

Other gene match

      
ANTIGEN VIEW
EDEM3-001
EDEM3-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.