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PLA2G4A

ANTIBODY INFORMATION
 
 

Antibody HPA050062

  

Antibody HPA054206

  

Antibody CAB010050

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA050062   HPA054206   sc-438  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   13   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA050062

  

Antibody HPA054206

  

Antibody CAB010050

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA050062

  

Antibody HPA054206

  

Antibody CAB010050

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to cytosol.   Immunofluorescent staining of human cell line HeLa shows localization to cytosol.    

Antibody dilution

 1:122   1:8    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA050062

  

Antibody HPA054206

  

Antibody CAB010050

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human parathyroid gland shows moderate positivity in glandular cells.   Immunohistochemical staining of human parathyroid gland shows moderate positivity in glandular cells.   Immunohistochemical staining of human parathyroid gland shows strong positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 49 cell types		   RNA: detected in 36 tissues
Protein: detected in 48 cell types		   RNA: detected in 36 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:18   1:40  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA050062

  

Antibody HPA054206

  

Antibody CAB010050

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 85.2   85.2   85.2  

Antibody dilution

 1:610   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA050062

  

Antibody HPA054206

  

Antibody CAB010050

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:4850   1:600    

ANTIGEN INFORMATION
 
 

Antibody HPA050062

  

Antibody HPA054206

  

Antibody CAB010050

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 82   91    

Antigen sequence

 
TVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENIT
TKHIVSNDSSDSDDESHEPKGTENEDAGSDYQ
  
PRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNI
DVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSK
  

											 
																				

																		

										
											
Matching transcripts

												
																					PLA2G4A-001 - ENSP00000356436 [100%] 

											 
																						PLA2G4A-001 - ENSP00000356436 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PLA2G4A-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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