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SDHB

ANTIBODY INFORMATION
 
 

Antibody HPA002868

  

Antibody CAB009822

  

Antibody CAB068233

  

Antibody CAB068234

  

Antibody CAB068235

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002868   sc-25851   AMAb90705   AMAb90706   AMAb90708  

Host species

 Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 3   4   13   13   13  

References

 17          

VALIDATION SUMMARY
 
 

Antibody HPA002868

  

Antibody CAB009822

  

Antibody CAB068233

  

Antibody CAB068234

  

Antibody CAB068235

  
Immunocytochemistry

Supported          
Immunohistochemistry

Supported   Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported   Supported  
Protein array

Approved          

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002868

  

Antibody CAB009822

  

Antibody CAB068233

  

Antibody CAB068234

  

Antibody CAB068235

  

Standard validation

 Supported          

         

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, plasma membrane & mitochondria.          

Antibody dilution

 1:34          

Literature conformity

 The subcellular location is supported by literature.          

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002868

  

Antibody CAB009822

  

Antibody CAB068233

  

Antibody CAB068234

  

Antibody CAB068235

  

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human liver shows strong positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human kidney shows strong positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 72 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:100   1:500   1:500   1:1400  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002868

  

Antibody CAB009822

  

Antibody CAB068233

  

Antibody CAB068234

  

Antibody CAB068235

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

         

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 31.6   31.6, 22.7, 14.1   31.6, 22.7, 14.1   31.6, 22.7, 14.1   31.6, 22.7, 14.1  

Antibody dilution

 1:250   1:500   1:1000   1:1000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA002868

  

Antibody CAB009822

  

Antibody CAB068233

  

Antibody CAB068234

  

Antibody CAB068235

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		          

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.          

Antibody dilution

 1:3000          

ANTIGEN INFORMATION
 
 

Antibody HPA002868

  

Antibody CAB009822

  

Antibody CAB068233

  

Antibody CAB068234

  

Antibody CAB068235

  

Antigen

 Recombinant protein fragment   Recombinant protein   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 109          

Antigen sequence

 
EGKQQYLQSIEEREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQ
AYRWMIDSRDDFTEERLAKLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIA
EIKKMMATY
  

											 
																						

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					SDHB-001 - ENSP00000364649 [100%] 

											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SDHB-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002868

												
										 
																				
											
											
											
Antibody CAB009822

												
										 
																				
											
											
											
Antibody CAB068233

												
										 
																				
											
											
											
Antibody CAB068234

												
										 
																				
											
											
											
Antibody CAB068235

												
										 
																		

																	

										
											
1.

												
																						An immunohistochemical procedure to detect patients with paraganglioma and phaeochromocytoma with germline SDHB, SDHC, or SDHD gene mutations: a retrospective and prospective analysis

	van Nederveen FH et al

	Lancet Oncol 2009;10(8):764-71

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						The Warburg effect is genetically determined in inherited pheochromocytomas

	Favier J et al

	PLoS One 2009;4(9):e7094

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						SDHA is a tumor suppressor gene causing paraganglioma

	Burnichon N et al

	Hum Mol Genet 2010;19(15):3011-20

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						A non-catecholamine-producing sympathetic paraganglioma of the spermatic cord: the importance of performing candidate gene mutation analysis

	Alataki D et al

	Virchows Arch 2010;457(5):619-22

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
5.

												
																						SDHB immunohistochemistry: a useful tool in the diagnosis of Carney-Stratakis and Carney triad gastrointestinal stromal tumors

	Gaal J et al

	Mod Pathol 2011;24(1):147-51

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
6.

												
																						Sumo1-ylation of human spermatozoa and its relationship with semen quality

	Marchiani S et al

	Int J Androl 2011;34(6 Pt 1):581-93

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
7.

												
																						Mitochondrial function and content in pheochromocytoma/paraganglioma of succinate dehydrogenase mutation carriers

	Rapizzi E et al

	Endocr Relat Cancer 2012;19(3):261-9

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
8.

												
																						Succinate dehydrogenase (SDH) D subunit (SDHD) inactivation in a growth-hormone-producing pituitary tumor: a new association for SDH?

	Xekouki P et al

	J Clin Endocrinol Metab 2012;97(3):E357-66

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
9.

												
																						Analysis of all subunits, SDHA, SDHB, SDHC, SDHD, of the succinate dehydrogenase complex in KIT/PDGFRA wild-type GIST

	Pantaleo MA et al

	Eur J Hum Genet 2014;22(1):32-9

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
10.

												
																						Integrated genomic study of quadruple-WT GIST (KIT/PDGFRA/SDH/RAS pathway wild-type GIST)

	Nannini M et al

	BMC Cancer 2014;14:685

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
11.

												
																						Paraganglioma and pheochromocytoma upon maternal transmission of SDHD mutations

	Bayley JP et al

	BMC Med Genet 2014;15:111

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
12.

												
																						A MEN1 syndrome with a paraganglioma

	Jamilloux Y et al

	Eur J Hum Genet 2014;22(2):283-5

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
13.

												
																						SDHB/SDHA immunohistochemistry in pheochromocytomas and paragangliomas: a multicenter interobserver variation analysis using virtual microscopy: a Multinational Study of the European Network for the Study of Adrenal Tumors (ENS@T)

	Papathomas TG et al

	Mod Pathol 2015;28(6):807-21

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
14.

												
																						C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization

	Desmurs M et al

	Mol Cell Biol 2015;35(7):1139-56

			Application: WB				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
15.

												
																						Aberrant DNA hypermethylation of SDHC: a novel mechanism of tumor development in Carney triad

	Haller F et al

	Endocr Relat Cancer 2014;21(4):567-77

			Application: WB				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
16.

												
																						Deciphering the molecular basis of invasiveness in Sdhb-deficient cells

	Loriot C et al

	Oncotarget 2015;6(32):32955-65

			Application: WB				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
17.

												
																						Tyrosine kinase receptors as molecular targets in pheochromocytomas and paragangliomas

	Cassol CA et al

	Mod Pathol 2014;27(8):1050-62

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.