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Immunohistochemical staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.
Expression
RNA: detected in 28 tissues Protein: detected in 15 cell types
RNA: detected in 28 tissues Protein: detected in 4 cell types
RNA: detected in 28 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1800
1:50
1:2000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Supported
Figure description
Distinct and selective staining in endothelial cells, observed in the whole brain.
WESTERN BLOT
Antibody HPA031345
Antibody HPA058494
Antibody CAB002759
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.1
54.1
54.1, 15.2, 12.9
Antibody dilution
1:250
1:80
1:500
PROTEIN ARRAY
Antibody HPA031345
Antibody HPA058494
Antibody CAB002759
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
