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ANTIBODY INFORMATION
 
 

Antibody HPA051282

  

Antibody HPA055838

  

Antibody CAB001515

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   GeneTex
  

Product name

 HPA051282   HPA055838   GTX21877  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 13   12   2  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA051282

  

Antibody HPA055838

  

Antibody CAB001515

  
Immunocytochemistry

 

Validated
   Supported  
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051282

  

Antibody HPA055838

  

Antibody CAB001515

  

Formal validation: Tagged protein

  
Validated

Antibody staining overlaps with GFP tagged protein		    

     

Figure description

   Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & vesicles and GFP expression in cytoplasm.    

Antibody dilution

   1:55    
 

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.   Immunofluorescent staining of human cell line A-431 shows localization to vesicles.  

Antibody dilution

   1:55   1:2000  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051282

  

Antibody HPA055838

  

Antibody CAB001515

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA055838 across 76 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA051282 across 76 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 8 cell types		   RNA: detected in 37 tissues
Protein: detected in 9 cell types		   RNA: detected in 37 tissues
Protein: detected in 29 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:6000   1:2000   1:16000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051282

  

Antibody HPA055838

  

Antibody CAB001515

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 59.8   59.8   59.8  

Antibody dilution

 1:360   1:270   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051282

  

Antibody HPA055838

  

Antibody CAB001515

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2850   1:2200    

ANTIGEN INFORMATION
 
 

Antibody HPA051282

  

Antibody HPA055838

  

Antibody CAB001515

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 85   87    

Antigen sequence

 
ADSRDPASDQMQHWKEQRAAQKADVLTTGAGNPVGDKLNVITVGPRGPLL
VQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFE
  
NTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNF
TEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHL
  

											 
																				

																		

										
											
Matching transcripts

												
																					CAT-001 - ENSP00000241052 [100%] 

											 
																						CAT-001 - ENSP00000241052 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

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