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Immunofluorescent staining of human cell line PC-3 shows localization to mitochondria.
Antibody dilution
1:44
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA036243
Antibody HPA044580
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
Antibody dilution
1:15
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036243
Antibody HPA044580
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407350)
Target mass (kDa)
36.2
36.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA036243
Antibody HPA044580
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
