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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA001578 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA000926 across 46 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human small intestine shows distinct positivity in plasma.
Immunohistochemical staining of human blood vessel shows distinct positivity in blood plasma.
Expression
RNA: detected in 12 tissues Protein: detected in 29 cell types
RNA: detected in 12 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA000926
Antibody HPA001578
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424544)
Target mass (kDa)
67
67
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA000926
Antibody HPA001578
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
