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XPO5

ANTIBODY INFORMATION
 
 

Antibody HPA018402

  

Antibody HPA023959

  

Antibody HPA029909

  

Antibody HPA029910

  

Antibody CAB012357

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA018402   HPA023959   HPA029909   HPA029910   31351  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

          

Released in version

 4   5   6   6   4  

References

          

VALIDATION SUMMARY
 
 

Antibody HPA018402

  

Antibody HPA023959

  

Antibody HPA029909

  

Antibody HPA029910

  

Antibody CAB012357

  
Immunocytochemistry


Validated

Validated
  

Validated
  

Validated
     Supported  
Immunohistochemistry

Supported   Supported  

Validated
  

Validated
   Supported  
Western blot


Validated
   Supported   Supported   Uncertain   Supported  
Protein array

Supported   Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA018402

  

Antibody HPA023959

  

Antibody HPA029909

  

Antibody HPA029910

  

Antibody CAB012357

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		          

 

Relative fluorescence intensity (RFI)

          

Figure description

 Nuclear region of segmented cells in 10x-images          

Antibody dilution

 1:55          
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA029909.
Antibody staining overlaps with antibody HPA023959.		  
Validated

Antibody staining overlaps with antibody HPA029909.
Antibody staining overlaps with antibody HPA018402.		  
Validated

Antibody staining overlaps with antibody HPA023959.
Antibody staining overlaps with antibody HPA018402.		      
 

Standard validation

 Supported   Supported   Supported     Supported  

         

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.     Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:54   1:50   1:50     1:25  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018402

  

Antibody HPA023959

  

Antibody HPA029909

  

Antibody HPA029910

  

Antibody CAB012357

  

Formal validation: Independent

    
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

   
 

  
 

    

Figure description

     Distribution of protein expression (antibody staining).
Pearson correlation with HPA029910 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA029909 across 45 cell lines.		    
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human testis shows strong positivity in seminiferus ducts.   Immunohistochemical staining of human testis shows distinct nuclear and cytoplasmic positivity in germ cells.   Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of cells outside the germinal center.   Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human kidney shows granular cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 54 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:50   1:250   1:35   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018402

  

Antibody HPA023959

  

Antibody HPA029909

  

Antibody HPA029910

  

Antibody CAB012357

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		          

         

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		          

Target mass (kDa)

 136.3, 28.3          

Loading control

 Total protein image          

Antibody dilution

 1:119          
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

         

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 136.3, 28.3   136.3   136.3   136.3   136.3, 28.3  

Antibody dilution

 1:250   1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA018402

  

Antibody HPA023959

  

Antibody HPA029909

  

Antibody HPA029910

  

Antibody CAB012357

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000   1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA018402

  

Antibody HPA023959

  

Antibody HPA029909

  

Antibody HPA029910

  

Antibody CAB012357

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 130   81   127   125    

Antigen sequence

 
ILGPLFTYLHMRLSQKWQVINQRSLLCGEDEAADENPESQEMLEEQLVRM
LTREVMDLITVCCVSKKGADHSSAPPADGDDEEMMATEVTPSAMAELTDL
GKCLMKHEDVCTALLITAFNSLAWKDTLSC
  
ASLVHLAFQIYEALRPRYLEIRAVMEQIPEIQKDSLDQFDCKLLNPSLQK
VADKRRKDQFKRLIAGCIGKPLGEQFRKEVH
  
MSFCVYSILGVVKRTCWPTDLEEAKAGGFVVGYTSSGNPIFRNPCTEQIL
KLLDNLLALIRTHNTLYAPEMLAKMAEPFTKALDMLDAEKSAILGLPQPL
LELNDSPVFKTVLERMQRFFSTLYENC
  
AGEWLKYQLSTFLDAGSVNSCSAVGTGEGSLCSVFSPSFVQWEAMTLFLE
SVITQMFRTLNREEIPVNDGIELLQMVLNFDTKDPLILSCVLTNVSALFP
FVTYRPEFLPQVFSKLFSSVTFETV
  

											 
																				

																		

										
											
Matching transcripts

												
																					XPO5-001 - ENSP00000265351 [100%] 
XPO5-015 - ENSP00000387384 [92%] 

											 
																						XPO5-001 - ENSP00000265351 [100%] 

											 
																						XPO5-001 - ENSP00000265351 [100%] 

											 
																						XPO5-001 - ENSP00000265351 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
XPO5-001
XPO5-015
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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