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SLC35B3

ANTIBODY INFORMATION
 
 

Antibody HPA054661

  

Antibody HPA057801

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA054661   HPA057801  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA054661

  

Antibody HPA057801

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA054661

  

Antibody HPA057801

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 48 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:700   1:500  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA054661

  

Antibody HPA057801

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 44.6   44.6  

Antibody dilution

 1:300   1:310  

PROTEIN ARRAY
 
 

Antibody HPA054661

  

Antibody HPA057801

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2400   1:3650  

ANTIGEN INFORMATION
 
 

Antibody HPA054661

  

Antibody HPA057801

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 23   68  

Antigen sequence

 
VIGNVQEKAMKLHNASNSEMVLY
  
QQAKDIQNITVQETNKNNSESIECSKITMDLKFNNSRKYISITVPSKTQT
MSPHIKSVDDVVVLGMNL
 

Matching transcripts

 SLC35B3-001 - ENSP00000368981 [100%]
  SLC35B3-001 - ENSP00000368981 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC35B3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.