RRBP1 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

RRBP1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA009026

Antibody HPA011924

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA009026

HPA011924

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA009026

Antibody HPA011924

Immunocytochemistry

Immunohistochemistry

Western blot

Uncertain

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA009026

Antibody HPA011924

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA011924.

Validated

Antibody staining overlaps with antibody HPA009026.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.

Antibody dilution

1:667

1:91

Literature conformity

The subcellular location is supported by literature.

Validation: Genetic

Supported

Significant downregulation 10-25% by one/two siRNA:s

Relative fluorescence intensity (RFI)

Figure description

Whole cells of segmented cells in 10x-images

Antibody dilution

1:200

IMMUNOHISTOCHEMISTRY

Antibody HPA009026

Antibody HPA011924

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA011924 across 45 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA009026 across 45 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA011924 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA009026 across 73 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 73 cell types

RNA: detected in 37 tissues
Protein: detected in 53 cell types

Retrieval

HIER pH6

Antibody dilution

1:10000

1:50

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA009026

Antibody HPA011924

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

152.5, 108.6, 102.8, 84.3

152.5, 108.6, 102.8, 84.3, 38.7

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA009026

Antibody HPA011924

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA009026

Antibody HPA011924

Antigen

Recombinant protein fragment

Length (aa)

107

124

Antigen sequence

ELHSKLQSSEAEVRSKCEELSGLHGQLQEARAENSQLTERIRSIEALLEA
GQARDAQDVQASQAEADQQQTRLKELESQVSGLEKEAIELREAVEQQKVK
NNDLREK

QLCLIEAQTMEALLALLPELSVLAQQNYTEWLQDLKEKGPTLLKHPPAPA
EPSSDLASKLREAEETQSTLQAECDQYRSILAETEGMLRDLQKSVEEEEQ
VWRAKVGAAEEELQKSRVTVKHLE

Matching transcripts

RRBP1-001 - ENSP00000354045 [100%]
RRBP1-002 - ENSP00000367044 [100%]
RRBP1-007 - ENSP00000401206 [100%]
RRBP1-201 - ENSP00000246043 [100%]
RRBP1-202 - ENSP00000367038 [100%]
RRBP1-203 - ENSP00000479709 [100%]

Other gene match

ANTIGEN VIEW

RRBP1-001	RRBP1-002	RRBP1-005	RRBP1-007	RRBP1-201	RRBP1-202	RRBP1-203

RELEVANT PUBLICATIONS

Antibody HPA009026

Antibody HPA011924

Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer
Telikicherla D et al
Clin Proteomics 2012;9(1):7

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.