Cell atlas - RRBP1 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	RRBP1
Gene description	Ribosome binding protein 1
Protein class	Plasma proteins Predicted intracellular proteins Predicted membrane proteins
Predicted localization	Intracellular,Membrane
Number of transcripts	8
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the endoplasmic reticulum.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Endoplasmic reticulum (validated)
siRNA	The location(s) are Supported based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA009026 , HPA011924
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the endoplasmic reticulum.

Main location

Endoplasmic reticulum (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA011924	A-431	Endoplasmic reticulum
HPA011924	U-2 OS	Endoplasmic reticulum
HPA011924	U-251 MG	Endoplasmic reticulum
HPA009026	A-431	Endoplasmic reticulum
HPA009026	U-2 OS	Endoplasmic reticulum
HPA009026	U-251 MG	Endoplasmic reticulum

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA009026

siRNA validation

Supported: Significant downregulation 10-25% by one/two siRNA:s

Analysis performed in

Whole cells of segmented cells in 10x-images

siRNA 2

RFI

81%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail 10x	Thumbnail 40x	Antibody	Sample	Cell line
		HPA009026	siRNA 2	U-2 OS
		HPA009026	scrambled	U-2 OS

GENE INFORMATION
Gene name	RRBP1 (HGNC Symbol)
Synonyms	ES/130, hES
Description	Ribosome binding protein 1 (HGNC Symbol)
Entrez gene summary	This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]
Chromosome	20
Cytoband	p12.1
Chromosome location (bp)	17613678 - 17682295
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000125844 (version 83.38)
Entrez gene	6238
UniProt	Q9P2E9 (UniProt - Evidence at protein level)
neXtProt	NX_Q9P2E9
Antibodypedia	RRBP1 antibodies

PROTEIN BROWSER

RRBP1-001	RRBP1-002	RRBP1-003	RRBP1-005	RRBP1-007	RRBP1-201	RRBP1-202	RRBP1-203

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
RRBP1-001 ENSP00000354045 ENST00000360807	Q9P2E9 [Direct mapping] Ribosome-binding protein 1 Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001649 [osteoblast differentiation] GO:0004872 [receptor activity] GO:0005783 [endoplasmic reticulum] GO:0005840 [ribosome] GO:0006412 [translation] GO:0015031 [protein transport] GO:0016020 [membrane] GO:0030176 [integral component of endoplasmic reticulum membrane] GO:0044822 [poly(A) RNA binding] Show all	977 aa 108.6 kDa	No	1
RRBP1-002 ENSP00000367044 ENST00000377813	A0A0A0MRV0 [Direct mapping] Ribosome-binding protein 1 Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0015031 [protein transport] GO:0030176 [integral component of endoplasmic reticulum membrane] Show all	1410 aa 152.5 kDa	No	1
RRBP1-003 ENSP00000381762 ENST00000398782	A2A2S5 [Direct mapping] Ribosome-binding protein 1 Show all	MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins SPOCTOPUS predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0015031 [protein transport] GO:0030176 [integral component of endoplasmic reticulum membrane] Show all	300 aa 31.7 kDa	No	0
RRBP1-005 ENSP00000478324 ENST00000470422	A0A087WU26 [Direct mapping] Ribosome-binding protein 1 Show all	Phobius predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		346 aa 38.7 kDa	No	0
RRBP1-007 ENSP00000401206 ENST00000455029	F8W7S5 [Direct mapping] Ribosome-binding protein 1 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005783 [endoplasmic reticulum] Show all	751 aa 84.3 kDa	No	0
RRBP1-201 ENSP00000246043 ENST00000246043	A0A0A0MRV0 [Direct mapping] Ribosome-binding protein 1 Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0015031 [protein transport] GO:0030176 [integral component of endoplasmic reticulum membrane] Show all	1410 aa 152.5 kDa	No	1
RRBP1-202 ENSP00000367038 ENST00000377807	Q9P2E9 [Direct mapping] Ribosome-binding protein 1 Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001649 [osteoblast differentiation] GO:0004872 [receptor activity] GO:0005515 [protein binding] GO:0005783 [endoplasmic reticulum] GO:0005840 [ribosome] GO:0006412 [translation] GO:0015031 [protein transport] GO:0016020 [membrane] GO:0030176 [integral component of endoplasmic reticulum membrane] GO:0044822 [poly(A) RNA binding] Show all	977 aa 108.6 kDa	No	1
RRBP1-203 ENSP00000479709 ENST00000610403	A0A087WVV2 [Direct mapping] Ribosome-binding protein 1 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		934 aa 102.8 kDa	No	0