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Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in bone marrow poietic cells. Distinct cytoplasmic positivity is seen in megakaryocytes.
Expression
RNA: detected in 15 tissues Protein: detected in 22 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023985
Standard validation
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401635)
Target mass (kDa)
37.1
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA023985
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
ANTIGEN INFORMATION
Antibody HPA023985
Antigen
Recombinant protein fragment
Length (aa)
51
Antigen sequence
VRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTT
E
