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SYNE1

ANTIBODY INFORMATION
 
 

Antibody HPA019113

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019113  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

 1  

VALIDATION SUMMARY
 
 

Antibody HPA019113

  
Immunocytochemistry

Supported  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019113

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane.  

Antibody dilution

 1:200  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019113

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic and nuclear membranous positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:85  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019113

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 1011.1, 1005.2, 964.8, 191.5, 157.6, 111.4, 108.9  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA019113

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA019113

  

Antigen

 Recombinant protein fragment  

Length (aa)

 132  

Antigen sequence

 
SRDLESAMSRALPSEDEEGQDDKDFYLRGAVGLSGDHSALESQIRQLGKA
LDDSRFQIQQTENIIRSKTPTGPELDTSYKGYMKLLGECSSSIDSVKRLE
HKLKEEEESLPGFVNLHSTETQTAGVIDRWEL
 

Matching transcripts

 SYNE1-001 - ENSP00000356224 [100%]
SYNE1-005 - ENSP00000441052 [100%]
SYNE1-033 - ENSP00000356226 [100%]
SYNE1-201 - ENSP00000341887 [100%]
SYNE1-002 - ENSP00000396024 [85%]
SYNE1-006 - ENSP00000356220 [85%]
SYNE1-027 - ENSP00000346701 [85%]
 

Other gene match

  
ANTIGEN VIEW
SYNE1-001
SYNE1-002
SYNE1-005
SYNE1-006
SYNE1-027
SYNE1-033
SYNE1-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA019113

  

1.

 Mammalian sperm head formation involves different polarization of two novel LINC complexes
Göb E et al
PLoS One 2010;5(8):e12072
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.