Fields »
ABOUT
HELP
BLOG
PPFIA1

ANTIBODY INFORMATION
 
 

Antibody HPA008272

  

Antibody HPA042271

  

Antibody CAB017032

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA008272   HPA042271   2172  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   12   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA008272

  

Antibody HPA042271

  

Antibody CAB017032

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

  Uncertain   Uncertain  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008272

  

Antibody HPA042271

  

Antibody CAB017032

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites.    

Antibody dilution

 1:78   1:96    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008272

  

Antibody HPA042271

  

Antibody CAB017032

  

Standard validation

   Uncertain   Uncertain  

     

Figure description

   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.   Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 38 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:3000   1:1000  

Literature conformity

   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008272

  

Antibody HPA042271

  

Antibody CAB017032

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 135.8, 134   135.8, 134   135.8, 134, 42.2, 26, 17.6, 11.4, 10.8  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008272

  

Antibody HPA042271

  

Antibody CAB017032

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA008272

  

Antibody HPA042271

  

Antibody CAB017032

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 122   130    

Antigen sequence

 
HHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDG
VLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQM
KERLASLSSHVTELEEDLDTAR
  
QLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPM
ADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLA
NVAQAFESDADVSDGEDDRDTLLSSVDLLS
  

											 
																				

																		

										
											
Matching transcripts

												
																					PPFIA1-001 - ENSP00000253925 [100%] 
PPFIA1-002 - ENSP00000374198 [100%] 

											 
																						PPFIA1-001 - ENSP00000253925 [100%] 
PPFIA1-002 - ENSP00000374198 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PPFIA1-001
PPFIA1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.