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CCNB1

ANTIBODY INFORMATION
 
 

Antibody HPA030741

  

Antibody HPA061448

  

Antibody CAB000115

  

Antibody CAB003804

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Transduction laboratories
   Epitomics
  

Product name

 HPA030741   HPA061448   610220   1495-1  

Host species

 Rabbit   Rabbit   Mouse   Rabbit  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Supernatant  

Other gene match

        

Released in version

 12   16   1   2  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA030741

  

Antibody HPA061448

  

Antibody CAB000115

  

Antibody CAB003804

  
Immunocytochemistry


Validated

Validated
  

Validated

Validated
   Supported    
Immunohistochemistry

 

Validated
  

Validated
  

Validated
  
Western blot


Validated
   Supported   Supported   Uncertain  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030741

  

Antibody HPA061448

  

Antibody CAB000115

  

Antibody CAB003804

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		        

 

Relative fluorescence intensity (RFI)

        

Figure description

 Cytoplasmic region of segmented cells in 10x-images        

Antibody dilution

 1:116        
 

Formal validation: Tagged protein

  
Validated

Antibody staining overlaps with GFP tagged protein		      

       

Figure description

   Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm and GFP expression in cytoplasm.      

Antibody dilution

   1:200      
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA061448.		  
Validated

Antibody staining overlaps with antibody HPA030741.		      
 

Standard validation

 Supported   Supported   Supported    

       

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.   Immunofluorescent staining of human cell line A549 shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.    

Antibody dilution

 1:116   1:200   1:200    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030741

  

Antibody HPA061448

  

Antibody CAB000115

  

Antibody CAB003804

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  
 

 

  
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 

 

  
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

   Supported   Supported   Supported  

       

Figure description

   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in subset of glandular cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.  

Expression

   RNA: detected in 36 tissues
Protein: detected in 27 cell types		   RNA: detected in 36 tissues
Protein: detected in 25 cell types		   RNA: detected in 36 tissues
Protein: detected in 40 cell types		  

Retrieval

   HIER pH6   HIER pH9   HIER pH6  

Antibody dilution

   1:450   1:1000   1:50  

Literature conformity

   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030741

  

Antibody HPA061448

  

Antibody CAB000115

  

Antibody CAB003804

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		        

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		        

Target mass (kDa)

 48.3, 44.9, 44.1, 26.8        

Loading control

 Total protein image        

Antibody dilution

 1:259        
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 48.3, 44.9, 44.1, 26.8   48.3, 44.9, 44.1, 26.8   48.3, 44.9, 44.1, 26.8, 21.5   48.3, 44.9, 44.1, 26.8, 21.5  

Antibody dilution

 1:250   1:1320   1:500   1:1500  

PROTEIN ARRAY
 
 

Antibody HPA030741

  

Antibody HPA061448

  

Antibody CAB000115

  

Antibody CAB003804

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:15850      

ANTIGEN INFORMATION
 
 

Antibody HPA030741

  

Antibody HPA061448

  

Antibody CAB000115

  

Antibody CAB003804

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Synthetic peptide  

Length (aa)

 73   72      

Antigen sequence

 
PVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEE
DLCQAFSDVILAVNDVDAEDGAD
  
TRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQ
LQAKMPMKKEAKPSATGKVIDK
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CCNB1-001 - ENSP00000256442 [100%] 
CCNB1-002 - ENSP00000424588 [100%] 
CCNB1-003 - ENSP00000423387 [100%] 
CCNB1-005 - ENSP00000426092 [100%] 

											 
																						CCNB1-001 - ENSP00000256442 [100%] 
CCNB1-002 - ENSP00000424588 [100%] 
CCNB1-003 - ENSP00000423387 [100%] 
CCNB1-005 - ENSP00000426092 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CCNB1-001
CCNB1-002
CCNB1-003
CCNB1-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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